A Phylogenetic Study of Cycads (1915)(en)(5s) by Chamberlain C.J.

By Chamberlain C.J.

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Et al. (1999). DNA pooling identifies QTLs on chromosome 4 for general cognitive ability in children. Human Molecular Genetics, 8, 915-922. , Holland, M. , & Higuchi, R. (2000). High-throughput SNP allele-frequency determination in pooled DNA samples by kinetic PCR. Genome Research, 10, 258-266. , Craig, I. , e t al. DNApooling and dense marker maps: A systematic search for genes for cognitive ability. Neuroreport, 10, 843-848. , Hamshere, M. , et al. (2000). Cheap, accurate and rapid allele frequency estimation of single nucleotide polymorphisms by primer extension and DHPLC in DNA pools.

Recently, this approach has been modified such that both allele-specificprimers are combined in a single competition reaction (Craig, McClay, Plomin, & Freeman, 2000; McClay, Sugden, Koch, Higuchi, & Craig, 2002). 5). Allele-specific primers can also be adapted for use in real-time, or “kinetic,” PCR-particularly in the context of analyzing pooled DNA samples (Germer, Holland, & Higuchi, 2000). 4. Primer extension methodology using chain termination to detect single nucleotide polymorphisms (SNPs).

Thousands of genotypes on a single chip. New developments should soon enable chip technology t o be applied t o pooled DNA samples, thereby conferring the advantages of both multiple genotyping and high sample throughput. Other Strategies for Size Fractionating Alleles Generated by SNPs: M . 8. Single nucleotide polymorphism (SNP) detection via the “Invader” assay. In the primary reaction, two probes bind t o the target DNA, only if the sequence matches precisely that of the allele being followed will the ‘%ap”of the detection probe be displaced and cleaved.

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