A Research Annual by Kenneth R. Miller (Eds.)

By Kenneth R. Miller (Eds.)

As a self-discipline, mobilephone biology has classically been outlined via the limits which outline the boundaries of its pursuits. those barriers may possibly practice to situation -cell biologists learn fabric in the mobilephone - or they could follow to dimension - cellphone biologists research fabric above the extent of the macromolecule.
for a few years, the bounds of procedure enforced those obstacles as definitely as any customized of the self-discipline. Our lack of ability to enquire constructions which can now not be saw or molecules which may no longer be detected guaranteed that phone biology wouldn't move the limits which associated the telephone to different degrees of association. because the 20th century nears its shut, despite the fact that, the improvement of a tremendous diversity of instruments and methods, a few actual, a few chemical, a few organic, has replaced this example eternally. mobile biology this present day crosses the boundary, hyperlinks the molecule with the organelle, affiliates the mobile reaction with the

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And Sahyoun, N. (l985a) Nature (London) 317 546-549. Wolf. M.. Cuatrecasas, P.. and Sahyoun. N. (1985b) J. Biol. Chem. 260: 15718-15722. Wong. A. J.. Kiehart, D. P.. and Pollard. T. D. (1985) J. Biol. Chem. 260: 46-49. Woodget. J. R.. and Hunter, J. (1987) J. Biol. Chem. 262 4836-4843. THE STRUCTURE AND FUNCTION OF CELL ADHESION MOLECULES Bruce A. Cunningham OUTLINE I. 11. 111. IV. V. INTRODUCTION N-CAM A. Amino Acid Sequence B. mRNAs and the N-CAM Gene L-CAM AND RELATED MOLECULES Ng-CAM AND OTHER SECONDARY CAMS GENERAL CONSIDERATIONS REFERENCES Advances in Cell Biology, Volume 2, pages 25-54.

M.. and Sahyoun. N. (1986) J. Biol. Chem. 261: 13327-13332. , LeVine. May. W. Cuatrecasas. P.. and Sahyoun, N. (l985a) Nature (London) 317 546-549. Wolf. M.. Cuatrecasas, P.. and Sahyoun. N. (1985b) J. Biol. Chem. 260: 15718-15722. Wong. A. J.. Kiehart, D. P.. and Pollard. T. D. (1985) J. Biol. Chem. 260: 46-49. Woodget. J. R.. and Hunter, J. (1987) J. Biol. Chem. 262 4836-4843. THE STRUCTURE AND FUNCTION OF CELL ADHESION MOLECULES Bruce A. Cunningham OUTLINE I. 11. 111. IV. V. INTRODUCTION N-CAM A.

The variants are of two types: ( I ) age-dependent differences in an unusual polysialic acid moiety and (2) tissue-dependent differences in the types of polypeptide chain. , 1984). which themselves have no unusual features. , 1982; Finne et al.. , 1984); estimates indicate chain lengths with a minimum size of 10-1 5 N-acetylneuraminic acid monomers. The polysialic acid is labile and is probably polydisperse in amount and size. Because of the 30 BRUCE A. CUNNINGHAM large negative charge or polydispersity of the polysialic acid, embryonic N-CAM is detected on polyacrylamide gels as a broad zone ranging from 200 to 250 kDa (Figure I A).

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