Advances in Chromatography, Volume 34 by Phyllis R. Brown, Eli Grushka

By Phyllis R. Brown, Eli Grushka

This thirty fourth quantity examines topics resembling high-performance capillary electrophoresis; gasoline chromatography, matrix isolation, and infrared spectrometry; and statistical theories of height overlap in chromatography.

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Charged analytes can be separated in a gel in their native state, although the use of a detergent such as sodium dodecyl sulfate (SDS) to solubilize and concomitantly denature all proteins is more common. The SDS interacts with the peptide bonds of the sample proteins. 2 g. The proteins thus treated appear negatively charged regardless of their charge before solubilization. Since the SDSprotein complexes have nearly similar electrophoretic mobilities, all proteins are separated solely by size. Since the complexes are negatively charged, the injection is performed at the cathode and the detector installed on the anodic side of the capillary.

Katti and Georges A. Guiochon Problems in Aqueous Size Exclusion Chromatography Paul L. Dubin Chromatography on Thin Layers Impregnated with Organic Stationary Phases Jiri Gasparic Countercurrent Chromatography for the Purification of Peptides Martha Knight Boronate Affinity Chromatography Ram P. Singhal and S. Shyamali M. DeSilva Chromatographic Methods for Determining Carcinogenic Benz(c)-acridine Noboru Motohashi, Kunihiro Kamata, and Roger Meyer Volume 32 Porous Graphitic Carbon in Biomedical Applications Chang-Kee Lim Tryptic Mapping by Reversed Phase Liquid Chromatography Michael W.

I. , Wilmington, Delaware Christine L. D. Section Research Chemist, Agricultural Products Department, E. I. D. Associate Professor, Department of Chemistry, Oklahoma State University, Stillwater, Oklahoma Oscar W. Reif Institut für Technische Chemie, University of Hannover, Hannover, Germany Page v Contents of Volume 34 Contributors to Volume 34 iii Contents of Other Volumes ix 1. High-Performance Capillary Electrophoresis of Human Serum and Plasma Proteins Oscar W. Reif, Ralf Lausch, and Ruth Freitag 1 I.

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