Analytical Chemistry of Bacillus thuringiensis by Leslie A. Hickle, William L. Fitch

By Leslie A. Hickle, William L. Fitch

content material: Analytical chemistry of Bacillus thuringiensis : an outline / Leslie A. Hickle and William L. Fitch --
old elements of the quantification of the lively component percent for Bacillus thuringiensis items / George Tompkins, Reto Engler, Michael Mendelsohn, and Phillip Hutton --
Bioassay equipment for quantification of Bacillus thuringiensis [delta]-endotoxin / Clayton C. Beegle --
Specificity of insecticidal crystal proteins : implications for commercial standardization / R. Milne, A.Z. Ge, D. Rivers, and D.H. Dean --
In vitro analyses of Bacillus thuringiensis [delta]-endotoxin motion / George E. Schwab and Paul Culver --
id of entomocidal pollution of Bacillus thuringiensis by means of high-performance liquid chromatography / Takashi Yamamoto --
Characterization of parasporal crystal pollution of Bacillus thuringiensis Subspecies kurstaki traces HD-1 and NRD-12 : use of oligonucleotide probes and cyanogen bromide mapping / L. Masson, M. Bossé, G. Préfontaine, L. Péloquin, P.C.K. Lau, and R. Brousseau --
improvement of a high-performance liquid chromatography assay for Bacillus thuringiensis var. san diego [delta]-endotoxin / Leonard Wittwer, Denise Colburn, Leslie A. Hickle, and T.G. Sambandan --
Use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis to quantify Bacillus thuringiensis [delta]-endotoxins / Susan M. Brussock and Thomas C. Currier --
Quantitative immunoassay of insecticidal proteins in Bacillus thuringiensis items / R. Gene Groat, James W. Mattison, and Eric J. French --
The light-scattering characterization of [delta]-endotoxin construction in inclusion our bodies / Fritz S. Allen, Betty J.M. Hannoun, Tammy B. Hebner, and Kathryn Nette --
Quantification of Bacillus thuringiensis insect regulate protein as expressed in transgenic vegetation / Roy L. Fuchs, Susan C. MacIntosh, Duff A. Dean, John T. Greenplate, Frederick J. Perlak, Jay C. Pershing, Pamela G. Marrone, and David A. Fischhoff --
High-performance liquid chromatography research of 2 [beta]-exotoxins produced by way of a few Bacillus thuringiensis traces / Barry L. Levinson.

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Invert. Pathol. 1974, 24, 365-73. , Jr. Appl. Environ. Microbiol. 1985,50,737-42. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1990. 4. MILNE ET AL. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. I. J. Bacteriol. 1977, 130, 375-83. M. Biochem. J. 1980, 187, 457-65. , Jr. Can. J. Microbiol. 1988,34,740-47. W. ; Permagon: Toronto, 1985; Vol. 4. p. 279. ; Kuriyama, K. Comp. Biochem. Physiol. 1983, 76B, 29-34. R. Can. J. Zool. 1989, 67, 864-68. R. Pharmac.

Eur. J. Biochem. 1989, (in press) Wolfersberger, M. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1990. Chapter 5 In Vitro Analyses of Bacillus thuringiensis δ-Endotoxin Action George E. ) endotoxins as determined by in vitro experimentation. t. endotoxins. The use of cell culture and isolated tissue preparations, which comprise the primary model systems for in vitro experimentation, has certain limitations. In most instances, the insect cells used in tissue culture are not considered the primary target for endotoxin interactions, ie.

Thuringiensis were originally identified by Heimpel (4) as alpha-exotoxin (lecithinase C or phospholipase C); alpha-exotoxin (thermostable toxin orflyfactor); and delta-endotoxin (the crystal protein). The latter was originally defined as the crystal protein and thought to represent a unique toxin (4). , B. mori, M. , Anagasta kuehniella or Lymantria dispar. (5) The classification of insects according to their crystal susceptibility should be reconsidered as the spectrum of activity of B. thuringiensis insecticidal crystal protein become more available.

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