Capillary Electrophoresis: Principles and Practice by Reinhard Kuhn, Sabrina Hoffstetter-Kuhn
By Reinhard Kuhn, Sabrina Hoffstetter-Kuhn
Capillary electrophoresis (CE) is a brand-new analytical strategy with the aptitude of fixing many analytical separation difficulties very quickly and economically. this system provides new information regarding the investigated ingredients which can't simply be bought via different capability. CE has turn into a longtime strategy only in the near past, yet should be carried out in virtually each analytical laboratory in undefined, carrier devices and academia within the close to destiny. an important fields of CE program are pharmaceutical and biochemical examine and qc. The authors have exhaustive sensible event within the program of CE tools within the pharmaceutical and supply the reader with a finished remedy of this technique. the focus is on tips to resolve difficulties whilst using CE within the laboratory. Physico-chemical concept is simply handled intensive whilst essential to comprehend the underlying separation mechanisms so that it will resolve your difficulties on the analytical bench. An addendum comprises tables at the practise of buffers and instructed additional studying.
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Extra resources for Capillary Electrophoresis: Principles and Practice
Eq. 2-46 is not defined for Ilea being equal to - it or lower. Although highest resolutions are obtained if electroosmotic and electrophoretic mobilities are adjusted such that Eq. 2-47 is fulfilled. these separations need to run for a long time. In practice. 5 are not desired because of the time wasted. The resolution between two peaks in an electropherogram can be calculated as follows: (248) t1 t2 W1 W2 migration time of component I [s] migration time of component 2 [s] temporal peak width of component 1 [s] (see Fig.
Buffers containing high salt concentrations minimize adsorption of proteins to the silica by an ion exchange mechanism. 25 M) has proven to be superior to alkaline halogenides with respect to the prevention of adsorption and UV absorbance interferences . d. ll11) can also be employed. Using zwitterions instead of high concentrations of potassium sulfate is a similar approach to prevent adsorption. Zwitterions do not contribute significantly to conductivity even at high concentrations, but compete strongly for the active surface sites.
Moreover, according to Eq. 3-14, the temperature difference between the center of the capillary and the walls is proportional to the heat production and to the square of the inner diameter of the capillary. In this regard lower field strengths commonly yield better resolution than high field strengths although this contradicts the theory. Because heat is directly related to the conductance and the concentration, highly conductive electrolytes and high concentrations increase production of heat.