Chemokine Receptors by Richard Horuk (Eds.)

By Richard Horuk (Eds.)

Chemokines are significant mediators of immune cells and are enthusiastic about a variety of proinflammatory human ailments, together with rheumatoid arthritis, a number of sclerosis, and organ transplant rejection. It has lately been came across that their receptors are keen on HIV an infection. The characterization of those molecules and their receptors is hence of fundamental significance in figuring out a few human ailments and infections. This quantity and its spouse quantity 287 Chemokines offer complete experimental protocols utilized in this box of study.

Key Features
* Chemokine receptors
* Chemokines in disease
* sign transduction of chemokines

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Extra info for Chemokine Receptors

Example text

L. Sidman and P. Racik, Brain Res. 62, 1 (1973). 34 CHEMOKINERECEPTORS 131 hNT Neuron Cultures Because the human brain cell explants present many problems with respect to consistency of brain samples and reliability of explantation, we decided to use a different human brain cell culture system, the hNT neurons, to continue our studies, hNT neurons are derived from a proliferating cell line, the NTera2 cells, which are obtained from a clonal cell line (Ntera2/ clone D1) derived from a human embryonal carcinoma.

Despite consistent explantation and culture conditions, we have only been successful in establishing 4 out of 6 human fetal cultures. In the two failed cultures neurons formed small clusters, but did not begin to elaborate processes on day 4 or 5. After more time in culture, all cells developed a granular appearance consistent with necrosis and were discarded without further study. This variability in neuronal survival does not appear to correlate either with differences in fetal age at time of explantation or with differences in transit time before explantation.

Rubinstein, J. Barnwell, S. R. de Cordoba, and R. E. Rubinstein, J. Exp. Med. 166, 776 (1987). 14A. R. Glabinski, M. Tani, V. K. Tuohy, and R. M. Ransohoff, Methods Enzymol. 288, 182 (1997). 3. The fixative is removed after 1 hr, and the cells are carefully washed twice in PBS and then stored in PBS at 4 ° in an airtight container until the time of immunostaining (never store for longer than 2 to 3 weeks unless PBS is replaced because evaporation will alter osmolarity). 005% Triton X-100 (v/v) and 10% (v/v) serum from either goat or donkey (depending on the species of the secondary antibody).

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