Culture of Animal Cells: A Manual of Basic Technique and by R. Ian Freshney(auth.)
By R. Ian Freshney(auth.)
This is the 6th version of the major textual content within the simple method of mobilephone tradition, world wide. carefully revised, it positive factors updates on really expert strategies in stem mobilephone learn and tissue engineering; updates on molecular hybridization, somatic telephone fusion, hybridomas, and DNA move; new sections on vitrification and Organotypic tradition, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. it really is written for graduate scholars, learn and medical scientists, and technicians and laboratory managers in cellphone and molecular biology labs and genetics labs.
PowerPoint slides of the figures in addition to different supplementary fabrics can be found at a significant other website: www.wiley.com/go/freshney/cellculture
Chapter 1 creation (pages 1–10):
Chapter 2 Biology of Cultured Cells (pages 11–23):
Chapter three Laboratory layout, structure, and gear (pages 25–36):
Chapter four apparatus and fabrics (pages 37–56):
Chapter five Aseptic method (pages 57–70):
Chapter 6 safeguard, Bioethics, and Validation (pages 71–88):
Chapter 7 tradition Vessels and Substrates (pages 89–98):
Chapter eight outlined Media and vitamins (pages 99–114):
Chapter nine Serum?Free Media (pages 115–132):
Chapter 10 instruction and Sterilization (pages 133–162):
Chapter eleven basic tradition (pages 163–186):
Chapter 12 culture and cellphone traces (pages 187–206):
Chapter thirteen Cloning and choice (pages 207–225):
Chapter 14 mobilephone Separation (pages 227–237):
Chapter 15 Characterization (pages 239–268):
Chapter sixteen Differentiation (pages 269–278):
Chapter 17 Transformation and Immortalization (pages 279–297):
Chapter 18 illness (pages 299–315):
Chapter 19 Cryopreservation (pages 317–334):
Chapter 20 Quantitation (pages 335–364):
Chapter 21 Cytotoxicity (pages 365–381):
Chapter 22 really expert Cells (pages 383–432):
Chapter 23 Stem Cells, Germ Cells, and Amniocytes (pages 433–462):
Chapter 24 tradition of Tumor Cells (pages 463–479):
Chapter 25 Three?Dimensional tradition (pages 481–495):
Chapter 26 Scale?Up and Automation (pages 497–515):
Chapter 27 really expert thoughts (pages 517–532):
Chapter 28 education courses (pages 533–568):
Chapter 29 challenge fixing (pages 569–591):
Chapter 30 In end (page 593):
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Extra info for Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Sixth Edition
D) Hexagonal Microcarriers. Nunc Microhex Beads. ) Plate 18. Spheroids, Encapsulation, and Microcarriers. (a) (b) (b) Human Fetal Lung Epithelial Cells. Cells growing on filter with human fetal lung fibroblasts on other side of filter. 4x objective. Giemsa stained. (d) (c) (c, d) Lung Cell Coculture. Fetal lung epithelial cells and fibroblasts as in (b). (c) Focused on cells above filter. (d) Focused on cells below filter. 40x objective. Giemsa stained. (f) (e) (e) Section of Coculture. Section through filter with A549 cells and fibroblasts.
A) Dome Forming in Monolayer of Wil Lung Adenocarcinoma. Mosaic of CEA-positive and CEA-negative cells. Focused on top of dome. Anti-CEA immonoperoxidase stained. 40x objective. (b) Dome Forming in Monolayer of WIL Lung Adenocarcinoma. Mosaic of CEA-positive and CEA-negative cells. Focused on monolayer. Anti-CEA immonoperoxidase stained. 40x objective. 1 mm (c) A549 Lung Adenocarcioma Cells Growing on Matrigel. Upper, 4x objective, lower 10x; bright field, unstained. ) (d) A549 Lung Adenocarcinoma Cells.
F) Mycoplasma, High Power. Hoechst-stained mycoplasma infected cells under 100x oil-immersion objective. Plate 16. Examples of Contamination. (a) Dye Exclusion, Naphthalene Black. Hemocytometer slide 200-mm square with viable (unstained) and nonviable (blue stained) cells. 40x objective. Plate 17. Viability and Cytotoxicity. (i) (a) Transfected Mosaic Spheroids Derived from Human Glioma Cell Line MOG-G-UVW. The Spheroids, ranging in size from 100 to 500 µm diameter, are composed of mixtures of cells transfected with the GFP gene (green) and cells transfected with the NAT gene (red).