Enzymes in Carbohydrate Synthesis by Mark D. Bednarski and Ethan S. Simon (Eds.)

By Mark D. Bednarski and Ethan S. Simon (Eds.)

content material: Enzymes as catalysts in carbohydrate synthesis / Eric J. Toone and George M. Whitesides --
Microbial aldolases in carbohydrate synthesis / C.-H. Wong --
Use of glycosyltransferases in synthesis of unnatural oligosaccharide analogs / Ole Hindsgaul, Kanwal J. Kaur, Uday B. Gokhale, Geeta Srivastava, Gordon Alton, and Monica M. Palcic --
Use of glycosidases and glycosyltransferases within the synthesis of complicated oligosaccharides and their glycosides / K.G.I. Nilsson --
Glycosylation via use of glycohydrolases and glycosyltransferases in preparative scale / Peter Stangier and Joachim Thiem --
Use of glycosyltransferases for drug amendment / Daniel Gygax, Mario Hammel, Robert Schneider, Eric G. Berger, and Hiltrud Stierlin --
a few features of enzymic synthesis of oligosaccharides utilizing acceptor saccharides hooked up to polymer providers / Uri Zehavi and Joachim Thiem --
Galactose oxidase : chosen homes and artificial functions / Adam W. Mazur --
commercial software of adenosine 5'-triphosphate regeneration to synthesis of sugar phosphates / H. Nakajima, H. Kondo, R. Tsurutani, M. Dombou, I. Tomioka, and ok. Tomita.

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46. 47. 48. 49. 50. 51. ENZYMES IN CARBOHYDRATE SYNTHESIS Simon, E . ; Whitesides, G. M . J. Am. Chem. , 1989, 111, 8920. Bednarski, M . ; Crans, D. ; Simon. E . ; Stein, P. ; Whitesides, G. M . ; Schneider, M . Tetrahedron Lett. 1988, 29, 427. Hammond, S. M . ; Jansson, A. M . ; Larsson, L. ; Pring, B. ; Town, C. ; Ekström, Β. Nature 1987, 327, 730. Danishefsky, S. ; DeNinno, M . P. Angew. Chem. Int. Ed. Engl. 1987, 26, 15. Bednarski, M . ; Chenault, Η. ; Simon, E . ; Whitesides, G. M . J. Am.

H . Tetrahedron Lett. 1988, 29, 4645. ; Effenberger, F. Angew. Chem. Int. Ed. Engl. 1988, 27, 716. von der Osten, C. ; Sinskey, A. ; Barbas, C. , III; Pederson, R. L . ; Wang, Y. ; Wong, C. -H. J. Am. Chem. Soc. 1989, 111, 3924. Alefounder, P. ; Baldwin, S. ; Perham, R. ; Short, N. J. Biochem. J. 1989, 257, 529. Chen, Y. L . Metalloenzyme Chemistry, Master Thesis, Texas A & M University, 1989. von der Osten, C. ; Barbas, C. , III; Wong, C. - H . ; Sinskey, A. J. Molecular Microbiology 1989, 3, 1625.

Current methods for the preparation of dihydroxyacetone phosphate are from dihydroxyacetone by chemical or enzymatic synthesis, and from FDP by in situ enzymatic generation ((5). Dihydroxyacetone phosphate can also be replaced with dihydroxyacetone and catalytic amounts of inorganic arsenate (18). We have recently developed an improved procedure for the chemical synthesis which is outlined in Figure 6. The major improvement is the use of a trivalent phosphorylating reagent, dibenzyl-N, N- d i e t h y l phosphoramidite, in the presence of triazole or tetrazole.

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