Fluorescent Proteins I: From Understanding to Design by R. Nifosì, V. Tozzini (auth.), Gregor Jung (eds.)

By R. Nifosì, V. Tozzini (auth.), Gregor Jung (eds.)

Fluorescent proteins are in detail attached to analyze within the existence sciences. Tagging of gene items with fluorescent proteins has revolutionized all components of biosciences, starting from basic biochemistry to medical oncology, to environmental examine. the invention of the fairway Fluorescent Protein, its first, seminal software and the inventive improvement of a vast palette of fluorescence proteins of alternative colors, was once therefore recognized with the Nobel Prize for Chemistry in 2008.

Fluorescent Proteins I is dedicated to the fundamental photophysical and photochemical facets of fluorescent protein expertise. skilled specialists spotlight color tuning, the exploration of switching phenomena and respective tools for his or her research. The booklet offers an intensive figuring out of fundamental molecular methods permitting the layout of fluorescent proteins for particular applications.

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Tretyakova YA, Pakhomov AA, Martynov VI (2007) Chromophore structure of the kindling fluorescent protein asFP595 from Anemonia sulcata. J Am Chem Soc 129:7748–7749 50. Yampolsky IV, Remington SJ, Martynov VI, Potapov VK, Lukyanov S, Lukyanov KA (2005) Synthesis and properties of the chromophore of the asFP595 chromoprotein from Anemonia sulcata. Biochemistry 44(15):5788–5793 One-Photon and Two-Photon Excitation of Fluorescent Proteins 37 51. Sch€afer LV, Groenhof G, Klingen AR, Ullmann GM, Boggio-Pasqua M, Robb MA, Grubm€uller H (2007) Photoswitching of the fluorescent protein asFP595: mechanism, proton pathways, and absorption spectra.

The left-ordinate axis represents the two-photon cross section (s2) values in GM (Goeppert–Mayer), and the rightordinate axis represents the two-photon brightness, s2 Â ’ (’ being the fluorescence quantum yield), in GM. The bottom x-axis represents the laser wavelength used for excitation and the top xaxis represents the (one-photon) transition wavelength. The excitation and fluorescence emission intensities are shown in arbitrary units. From Drobizhev et al. [67] and for both neutral and anionic species (for instance, mKalama1 and mAmetrine contain the chromophore in the neutral state [21, 24]).

Nat Methods 4(9):741–746 108. Blab GA, Lommerse PHM, Cognet L, Harms GS, Schmidt T (2001) Two-photon excitation action cross-sections of the autofluorescent proteins. Chem Phys Lett 350:71–77 109. Heikal AA, Hess ST, Webb WW (2001) Multiphoton molecular spectroscopy and excitedstate dynamics of enhanced green fluorescent protein (EGFP): acid–base specificity. Chem Phys 274:37–55 110. Marchant JS, Stutzmann GE, Leissring MA, LaFerla FM, Parker I (2001) Multiphotonevoked color change of DsRed as an optical highlighter for cellular and subcellular labeling.

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