Groupes abstraits by De Seguir J.-A.

By De Seguir J.-A.

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Natl. Acad. Sci. USA 81, 4275–4279. McGhee, J. D. and von Hippel, P. H. (1975) Formaldehyde as a probe of DNA structure. I. Reaction with exocyclic amino groups of DNA bases. Biochemistry 14, 1281–1296. McGhee, J. D. and von Hippel P. H. (1975) Formaldehyde as a probe of DNA structure. II. Reaction with endocyclic imino groups of DNA bases. Biochemistry 14, 1297–1303. Orlando, V. and Paro, R. (1993) Mapping Polycomb-repressed domains in the bithorax complex using in vivo formaldehyde cross-linked chromatin.

1. as follows: 1. Supplement the nuclear digest buffer with 250 mM sucrose. 2. Digest K562 nuclei at 5 mg/mL (DNA) using 300 U micrococcal nuclease (MNase)/mg DNA at 20°C for 2–5 min. The length of time and level of enzyme will vary considerably depending on cell type (see Note 1). 5 mM spermidine for magnesium chloride in preparation and digestion buffers to stabilize the nuclei (15) and minimize endonuclease and protease digestion. 3. Salt Fractionation Active sequences in a micrococcal nuclease-generated chromatin preparation can be enriched by salt precipitation of the H1/H5-containing chromatin (16,17).

1999). A developmental switch in H4 acetylation upstream of Xist plays a role in X chromosome inactivation. EMBO J. 18, 2897–2907. 13. , Cooke, N. , and Liebhaber, S. A. (2000). Targeted recruitment of histone acetyltransferase activity to a locus control region. J. Biol. Chem. 275, 13,827–13,834. 14. , Liebhaber, S. , and Cooke, N. E. (2000). Patterns of histone 14 acetylation suggest dual pathways for gene activation by a bifunctional locus control region. EMBO J. 19, 6814–6822. 15. Hewish, D.

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