Immunocytochemistry and Related Techniques by Adalberto Merighi, Laura Lossi

By Adalberto Merighi, Laura Lossi

This quantity provides a set of protocols for immunocytochemical research of neurons and neural networks. Chapters specialise in immunocytochemical localization at mild and digital degrees, biochemical characterization, and sensible research in vivo or ex vivo through novel kinds of microscopy, in addition to protocols for improvement and construction of genetic probes. Written for the preferred Neuromethods sequence, chapters comprise the type of aspect and key implementation suggestion that guarantees profitable leads to the laboratory.

Essential and authoritative, Immunocytochemistry and comparable Techniques is meant for a wide viewers of scientists, together with histologists, biochemists, mobile and molecular biologists, electrophysiologists which are at the moment lively within the box or are prepared to go into the interesting zone of neuroscience research.

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91. 92. 93. 94. 31 microscopy. J Histochem Cytochem 45:947–956 Takizawa T, Robinson JM (2000) FluoroNanogold is a bifunctional immunoprobe for correlative fluorescence and electron microscopy. J Histochem Cytochem 48: 481–486 Robinson JM, Takizawa T (2009) Correlative fluorescence and electron microscopy in tissues: immunocytochemistry. J Microsc 235:259–272 Takizawa T, Robinson JM (2012) Correlative fluorescence and transmission electron microscopy in tissues. Methods Cell Biol 111:37–57 Kirizs T, Kerti-Szigeti K, Lorincz A et al (2014) Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells.

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Merighi A, Polak JM (1993) Post-embedding immunogold staining. In: Cuello AC (ed) Immunohistochemistry, 2nd edn. Wiley, London, New York, pp 229–264 49. Aimar P, Lossi L, Merighi A (1997) Immunogold labeling for transmission electron microscopy: exploring new frontiers. Cell Vision 4:394–407 50. Aimar P, Lossi L, Merighi A (2002) Immunocytochemical labeling methods and related techniques for ultrastructural analysis of neuronal connectivity. In: Merighi A, Carmignoto G (eds) Cellular and molecular methods in neuroscience research.

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