Instrumental liquid chromatography: a practical manual on by N. A. Parris

By N. A. Parris

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J. Chromatogr. , 1 5 (1977) 303-316. A. W. J. DeJong, H. Poppe and J. C. Kraak, J. , 148 (1978) 127-141. A. T. James and A. J. P. Martin, J. , 5 0 (1952) 6 7 9 - 6 9 0 . R. M. McCormick and B. L. Karger, Anal. , 52 (1980) 2249-2257. C. R. Wilke and P. Chang, J. A m . Inst. Chem. , 1 (1955) 264-270. H. R . Bruins, Coefficients of Diffusion in Liquids, International Critical Tables, Vol. 5, McGraw-Hill, New York, 1929, pp. 63-76. G. J. Kennedy and J. H. Knox, J. , 1 0 (1972) 549-556. J. H. Knox, J.

29). Currently, a practical velocity which may be considered typical is 10mm/sec, although, as indicated in Fig. 7, using columns containing fine particles, higher velocities could be employed without significant loss of efficiency. ) for the 10- and 5-pmdiameter supports, respectively. ), would be necessary. , 50-250mm in length, packed with these fine materials. This choice reduced the inlet pressure requirements for a given velocity and the overall void time, essentially in proportion t o the reduction in column length.

J. Kennedy and J. H. Knox, J. , 1 0 (1972) 549-556. J. H. Knox, J. Chromatogr. , 1 5 (1977) 352-364. This Page Intentionally Left Blank Chapter 3 The chromatographic support and column INTRODUCTION Of all the factors contributing to the advances in the practice of LC in recent years, the characterisation of the influence of the chromatographic support and the subsequent development of specialised materials must be regarded as the most important. LC has traditionally been a slow technique, offering only a limited separating power.

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