Lipid analysis and lipidomics : new techniques and by Magdi M Mossoba
By Magdi M Mossoba
Content material: procedure and purposes in lipid research / NIls Hinrichsen and Hans Steinhart -- an outline of contemporary mass spectrometry tools within the toolbox of lipid chemists and biochemists / R. Moreau -- worldwide mobile lipidome analyses via shotgun lipidomics utilizing multidimensional mass spectrometry / X. Han and R.W. Gross -- LC/MS and chiral separation / A. Kuksis and Y. Itabashi -- LC/MS and lipid oxidation / A. Kuksis and O. Sjovall -- Structural research of unsaturated fatty acid methyl ester isomers with acetonitrile covalent adduct chemical ionization (CACI) / J.T. Brenna -- contemporary advances in silver-ion HPLC using acetonitrile in hexane as solvent: temperature results on lipid elution orders/resolution / R.O. Adlof -- research of trans-18:1 fatty acids via silver ion HPLC / P. Delmonte, J.K.G. Kramer, and M.P. Yurawecz -- High-performance size-exclusion chromatography for lipid research in natural media / G. Márquez-Ruiz and M.C. Dobarganes -- Lipid separations utilizing packed-column supercritical fluid chromatography / D.G. Hayes --TLC-FID with precise connection with marine lipids and different high-molecular-weight natural compounds / R.G. Ackman and A. Timmins -- quick GC for mobile reputation research of micro organism / J.S. patron. Use of mobile fatty acids to spot food-borne pathogens by means of infrared spectroscopy and capillary gasoline chromatography / M.M. Mossoba and S.F. Al-Khaldi -- program of FT-NIR for fast choice of the trans fatty acid composition in fat and oils / H. Azizian and J.K.G. Kramer -- Infrared spectroscopy and partial least sq. calibration within the simultaneous quantification of remoted trans and conjugated linoleic acids / A.A. Christy -- research of protein-lipid interactions by way of vibrational spectroscopy / G. Meng, N.K. Howell, and E.C.Y. Li-Chan -- fats replacers: an outline / W.E. Artz, S.M. Mahungu, and S.L. Hansen -- Phospholipids: constructions and physicochemical actions / M.C. Erickson -- Waxes and sterols: constructions and chemistry / E.J. Parish and A.D. Bell
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Extra info for Lipid analysis and lipidomics : new techniques and applications
Given the physical factors affecting the process of selective ionization of analytes in the electrospray ion source and the different electrical properties of each of the lipid classes, we recognized that the electrospray ion source can be used to resolve lipid classes in a crude lipid extract into different categories based on the intrinsic electrical properties of each lipid class. Such a separation is analogous to the use of an ion-exchange column to separate individual lipid classes [as was employed previously (17)] before mass spectrometry in the initial mass spectrometric investigations (12,13).
Many types of “soft” ionization techniques have been developed and employed for lipid research (Table 1). Figure 4 illustrates the type of spectra that are generated when a larger lipid (a sophorolipid, molecular weight 688) is analyzed by a “hard” ionization method (LC-MS-EI) and also by a “soft” ionization method involving atmospheric pressure chemical ionization (LC-MS-APCI). The mechanism of soft ionization by APCI will be described in detail in a later section of this chapter. Note that the “hard” method produced many small fragments and no molecular ions and the “soft” method produced larger fragments and a discernible [M+H]+ ion.
Because the ions have the same energy, yet different mass, they reach the detector at different times (Fig. 11C). In tandem mass spectrometry (sometimes called MS-MS or MSn), sample ions are first separated by size in one mass analyzer; then an ion of a particular size is chosen and introduced into a collision cell (Fig. 11D). In the collision cell, the selected ion collides with a collision gas (typically argon or helium) resulting in fragmentation. The resulting fragment ions then enter a second mass analyzer, in which the fragment ions are separated by size and then detected.