Metallomics: Analytical Techniques and Speciation Methods by Bernhard Michalke

By Bernhard Michalke

Most up-to-date advancements, new insights and information derived from speciation research in a single detailed compilation: The reader will get accustomed to appropriate instrumental in addition to program features of metallomics methods, paving the line to realizing destiny, pathway, and motion of metals in atmosphere and organisms. Upon an introductory bankruptcy on analytical tools and methods, the entire bandwidth of functions is mentioned. professional bankruptcy authors solid spotlights on fresh subject matters resembling metallomics purposes to environmental and foodstuff reviews in addition to biology and medication. unique chapters take care of the influence of manganese and iron on neurodegeneration, and the effect of nanoparticles on overall healthiness.

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Example text

Although such a book can never consider entirely all aspects in its respective field, I think this book actually covers a comprehensive and broad range of relevant topics in the metallomics field with the intended focus on novel techniques and elemental speciation. I hope it will sensitize the readers for the need of thinking in “elemental species” rather than in “elements” and it will draw the readers' attention also to the analytical challenge and complexity. It will be a success of this book if – aside from distributing the recent knowledge about metallomics – the awareness for appropriate and careful sample handling and analysis will also grow, which is necessary to maintain original and quantitative species information in metallomics.

6 An example of separation of arsenolipids by reversed-phase HPLC/ICPMS. 1% formic acid (gradient: 0–15 min from 10% ethanol to 95%; 15–30 min 95% ethanol) was used. 4 ml min−1, column temperature 40 °C, and injection volume 20 µl. 7 Instrumental setup for the analysis of arsenolipids by HPLC/ICPMS/ESMS. The HPLC system consisting of a reversed-phase column employing either water/methanol or water/ethanol gradients is coupled simultaneously to an ICPMS used as arsenic-selective detector at m/z 75 for quantification and an ESMS for molecular detection of arsenolipids.

Adapted with permission from [21]. 3 Workflow and analytical techniques in metallothiolomics. The metallothiolome is a subgroup of the metallome. 1 Comparison of the flow profile of the EOF (a) and the laminar flow during HPLC separations (b) and its influence on the resulting peak shape. 2 Overview about ICP-MS detectable elements. (Adapted from Ref. 3 Schematic overview of an ICP-MS/MS with an octopole collision and reaction cell (Agilent 8800). 4 Interference-free detection of arsenic using ICP-MS/MS operated in the O2 mode.

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