Methods of Biochemical Analysis, Volume 8 by David Glick

By David Glick

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Extra resources for Methods of Biochemical Analysis, Volume 8

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I 5 x' A. T. JAMES 36 TABLE IX Comparison of Resulta Obtained with a Standard Mixture of Fatty Acids on Apieron L at 200°C. 1 3 4 % shown by peak area measurement by triangulation. ) No loss of saturated or unsaturated acids could be demonstrated with times of passage greater than 4 hours. The lower temperature of 180°C. has another advantage. The separation factors are greater than at higher temperatures and the specific retention volumes are still considerably lower than with Apiezon L columns (see Table X), so that separations are still rapid.

Pressure, 14 cm. /min. Peak identification: (1) air, (2) formic, (3) acetic, (4) propionic, (5) isobutyric, (6) n-butyric, (7) a-methylbutyric and isovaleric, ( 8 ) n-valeric, (9) 3-methylbutyric, (10) isocaproic, (11) n-caproic. w + -4 c z 8 l1 K 2 (0 5u FE: s A. T. JAMES 32 TABLE VII Retention Volumes of Methyl Esters of Saturated Fatty Acids from Formic to n-Caproic Relative to Methyl n-Butyrate in a Variety of Stationary Phases at Two Temperatures Stationary phase At 100°C. 6"c. 36 3. Acids of Chain Length C&,, This group comprises most of the acids of present biological interest, but the great variety of saturated and unsaturated acids in this group has so far prevented complete resolution on one column.

12. Separation of methyl esters of the short chain (C,-C,) acids. 5 cm. /min. Peak identification: (1) air, (2) formic, (3) methanol, (4) acetic, (5) propionic, (6) isobutyric, (7) trimethylacetir, (8) n-butyrir, (9) isovalerir, (10)n-valeric, (11) isocaproic, (12) n-caproic. I Fig. 11. Separation of methyl eaters of the short chain (C,-C,) acids. ; pressure, 14 cm. /min. Peak identification: (1) air, (2) formic, (3) acetic, (4) propionic, (5) isobutyric, (6) n-butyric, (7) a-methylbutyric and isovaleric, ( 8 ) n-valeric, (9) 3-methylbutyric, (10) isocaproic, (11) n-caproic.

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