Microfluidic Cell Culture Systems by unknown author

By unknown author

The fields of microfluidics and BioMEMS are considerably impacting phone biology examine and functions in the course of the software of engineering options to human sickness and illnesses. the scale of microfluidic channels are like minded to the actual scale of organic cells, and the various merits of microfluidics make it an enticing platform for brand new options in biology.

This new specialist reference applies the suggestions of microsystems to mobile tradition functions. The authors supply a completely sensible advisor to the foundations of microfluidic gadget layout and operation and their program to phone tradition concepts. The ensuing booklet is filled with thoughts and strategies that may be instantly deployed within the lab. both, the insights into telephone tradition functions will supply these thinking about conventional microfluidics and BioMEMS with an knowing of the explicit calls for and possibilities awarded by way of organic applications.

The target is to lead new and researchers and know-how builders to the real components and state-of-the-practice options that might increase the potency and cost in their applied sciences, units and biomedical items.

  • Provides insights into the layout and improvement of microfluidic platforms with a selected specialise in phone tradition applications
  • Focuses on innovations and methods for the layout and fabrication of microfluidic structures and units for telephone culture
  • Provides balanced insurance of microsystems engineering and bioengineering

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72] assembled five microfluidic layers constructed in a biodegradable substrate to create a 3-D tissue culture scaffold, which was then seeded with a high density of HepG2 cells that formed aggregates throughout the microchannels. The capacity for long-term viability was assessed by measuring albumin secretion rates. Kniazeva et al. [60] employed a similar approach to construct individual blood-gas subunits that could be stacked together to form a microfluidic respiratory assist device to study gas permeance in artificial lung applications.

The HMEC-1 cells lined the channels as a monolayer and expressed markers for CD31 and von Willebrand factor Cell expansion slowed after 10 days and parts of the capillary networks were covered by nearly confluent ECs within 14 days. 5 (Continued) 292 µl/h After 5 days of culture, seeded cells remained localized to the internal surfaces of collagen networks [34] N/A Development of functional blood vessels. Also promoted vascularization of the implanted tissues [76,77] N/A Prevascular networks composing of HUVECs were formed in all the triple layer constructs [62] Cells were embedded in the collagen hydrogel 4 µl/min [74] Human hepatocarcinoma cells (HepG2) Cells were cultured in the microchannels and incubated at 37 C in a 5% CO2 5À20 µl/min Hepatocyte carcinoma cells (HepG2) Cells were cultured in the microchannels and incubated at 37 C in a 5% CO2 Cells were seeded in 3-D constructs and then implanted in mice 280 µm/h (volumetric flow rate) N/A Viability of cells was maintained with channel diffusion after a week of culture, while the viability dropped without channel perfusion Without culture change cells rapidly died.

Ling et al. [40] demonstrated that flow networks embedded in agarose can efficiently provide nutrient and waste exchange for surrounding cells. Choi et al. , calcein-acetoxymethyl ester (AM)) delivery in cell-seeded alginate hydrogels, while Gillette et al. [53] demonstrated in situ collagen fiber assembly in a cell-seeded hydrogel. 1D) [48À51]. Although not fully 3-D, the ability to vary the depth enables construction of networks with architectures that more closely follow the scalings predicted by Murray’s law [57,58].

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