Modern Derivatization Methods for Separation Sciences by Toshimasa Toyo'oka

By Toshimasa Toyo'oka

Contains new derivatizing reagents now not lined in comparable ebook by means of Blau and Halket (Wiley 1993) and never present in the other books thus far. A box of accelerating value and importance in separation technological know-how. Concentrates on synthesis of derivatives for HPLC and Capillary Electrophoresis, options of serious curiosity within the pharmaceutical box.

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2 shows the chromatogram of amino acids (except tryptophan) in hydrolysate of milk-based infant formula. This method determines sulfurcontaining amino acids as stable cysteic acid and methionine sulfone using oxidation with performic acid and hydrolysis with HC1. The analysis of tryptophan includes alkaline hydrolysis with barium hydroxide followed by PITC derivatization, and applied for the analysis of infant formula [68]. Fig. 2. Chromatogram of PTC cysteic acid (CYSCOOH) and methionine sulfone (METONE) in performic acid plus 6 M HC1 hydrolysate of milk-based infant formula.

The method is based on the principle that fatty acids are extracted as ion-pairs with chloroform from the aqueous acetonitrile mobile phase after the post-column addition of aqueous methylene blue solution. The chloroform phase containing the ion-pairs is monitored with an absorbance detector at 651 nm. This method was applied for the analysis of orange juice, margarine and butter. The detection limits ranged from 26 to 83 ng, depending upon the acid. One problem associated specifically with the system as set up for fatty acids was the need routinely to backflush the detector cell with acetonitrile to remove deposits which caused fatty acid adsorption and thus loss of resolution and sensitivity.

1— Introduction Chemical compounds found in foods can be divided into two categories: essential nutrients that maintain human lives and substances which have unfavorable effects on man. The former includes saccharides, fats, amino acids, proteins, vitamins, and minerals. The latter contains environmental hazardous compounds such as organic mercury and PCB. Organochlorine and Edited by Toshimasa Toyo'oka: Modern Derivatization Methods for Separation Sciences (c) 1999 John Wiley & Sons Ltd. html 30/09/2003 Document Página 1 de 2 Page 14 organophosphorous pesticides, food additives such as antioxidants and preservatives, synthetic antibacterials and antibiotics used for drugs in feeds and animals, have been used for purposes of insecticide, preservation, disease control in the process of culturing, harvesting, and food preparation.

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