Near-Infrared Applications in Biotechnology (Practical by Ramesh Raghavachari

By Ramesh Raghavachari

This quantity explores advancements in thoughts in diagnostics, DNA sequencing, bioanalysis of immunoassays, and single-molecule detection. It promotes the dimension, identity, tracking, research, and alertness of near-infrared spectroscopy (NIR) to clinical and pharmaceutical advances. The textual content additionally considers noninvasive tools of NIR for profitable, reasonably-priced, and suggested diagnoses of illnesses.

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Biophys J 541577-586,1988. Orientationfactor in steady-stateandtime-resolvedresonance energy transfer measurements. Biochemistry 31:7939-7947, 1992. LJ Parkhurst, KM Parkhurst. Changes in the end-to-end distance distribution in an oligonucleotide following hybridization. SPIE 2137:475483, 1994. Kineticstudies by fluorescenceresonanceenergy transfer employing a double-labeled oligonucleotide: Hybridization to the oligonucleotide complement and to single-stranded DNA. Biochemistry 34:285-292, 1995.

S Rajendran, W Bujalowski. Complex of Escherickicr coli primary replicative helicase DnaB protein with a replication fork: Recognition and structure. Biochemistry 3 7 3 1 16-3 136, 1998. 82. Intermediatespecies possessing bent DNA are present along the pathway to formation of a final TBpTATA complex. J Mol Biol 289:1327-1341, 1999. This Page Intentionally Left Blank Probes: Dyes Fluorescing in the NIR Region Stefan Stoyanov University of Sofia, Sofia, Bulgaria 1. INTRODUCTION Dye research and application have always been dominated by the demands of textile chemistry.

For wobble within a cone and that for K~ = 2/3 as a function of the cone angle 8 . The top (A)and bottom (W) curves give the extreme valuesfor the same ratio based, respectively, on canonical structures C and A of Figure 7 and the indicated cone angle. Thus,for 8 = 60", for the 68% confidence region, the uncertainty in would be only 4%. As an example of theuse of anisotropydatatoestimateaconeangle, when a IO-mer oligoribonucleotide was labeled at the 3' oxygen with fluorescein isothiocyanate (nine single bonds from the dye to the 0 on the phosphate) andthecomplexwasbound to 40s ribosomes,thecalculatedconeangle (8) was 55" [72].

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