Neuroscience by T. Heinbockel

By T. Heinbockel

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5-mm-diameter borosilicate glass; tip diameter was 2–3 µm, tip resistance was 5–8 MΩ. Seal resistance was routinely >1GΩ and liquid junction potential was 9-10 mV; reported measurements were not corrected for this potential. Data were obtained using a Multiclamp 700B amplifier (Molecular Devices, Sunnyvale, CA). 1, Molecular Devices). Data were also collected through a Digidata 1440A Interface (Molecular Devices) and digitized at 10 kHz. Holding currents were generated under control of the Multiclamp 700B Commander.

Neuroscience 79:425-34. ISSN 0306-4522 Aroniadou-Anderjaska V, Ennis M, Shipley MT (1999). Current-source density analysis in the rat olfactory bulb: laminar distribution of kainate/AMPA and NMDA receptormediated currents. Journal of Neurophysiology 81:15-28.

2005 with permission of the Society for Neuroscience. Electrophysiological Recording and Imaging of Neuronal Signals in Brain Slices 41 4. Conclusion mGluR1 in mitral cells and mGluR5 in granule cells are expressed in higher density in the MOB than in most other regions of the brain suggesting a critical functional role for mGluRs in the operations of the MOB network. We described new findings demonstrating that the excitability of mitral cells and granule cells is directly and potently modulated via activation of mGluRs by endogenously released glutamate in MOB slices.

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