Physics Reports vol.90

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3. MICROSCOPIC KINETIC THEORIES 37 chromatography is a computer adaptation of a composite Poissonian stochastic process. It consists of generating a sequence of exponentially distributed fly times followed by exponentially distributed adsorption sojourn times. The column residence time histogram corresponds to the chromatogram. A finite number of adsorption sites are distributed along the column and they can allocate only a finite number of molecules. 51) where Nmol indicates the number of molecules injected into the column, Nsite is the number of sites in the column.

1. 2. 3. Conclusion: Where Do We Go Next? Science vs. 1. INTRODUCTION Liquid chromatography (LC) has gained a dominant position in the life sciences and has a strong impact on all facets of human life [1]. Primarily, LC serves as a key technology in the analysis and production of value added chemicals in particular of pharmaceuticals. Second, it enables monitoring of changes in our environment and provides efficient tools, such as those to control the emission of pollutants and determine their distribution and metabolism.

At constant volume flow rates, slight changes in column internal diameter result in large changes in linear velocity and consequently large changes in retention times. Another critical parameter of the column hardware is the filter material and fittings, which hold the microparticulate packing material inside the column. The filter needs to be chemically inert and mechanically stable. In addition, it should have an appropriate porosity and average pore size to prevent the particles moving out of the column and avoid becoming blocked.

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