Physics Reports vol.91

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The sample is placed in the tube, and a sheet of dialysis tubing is placed over the orifice of the tube. The cap is then carefully closed so that the dialysis tubing forms a barrier between the solution inside the tube and the outside environment. 1). Of course, the success of this method depends critically on forming a leakproof seal with the dialysis tubing. Volumes as small as 10 ~-tl can be effectively dialyzed in this way. Gel Filtration Chromatography An alternative method for removing small molecular weight species from protein solutions is the use of gel filtration chromatography (Cooper, 1977; Scopes, 1982).

Open the desiccator in the hood, and leave it open for a few minutes to eliminate silane vapors. 5. Rinse glassware with distilled water before drying or autoclaving and storage. NOTES 1. These silane liquids are flammable, and give off highly toxic vapors. Due caution should be exercised during this procedure. 2. Do not leave the vacuum pump connected to the desiccator with the stopcock open after the silane begins to boil. Doing so will evacuate the silane from the desiccator and thus minimize the effectiveness of the treatment.

Do not leave the vacuum pump connected to the desiccator with the stopcock open after the silane begins to boil. Doing so will evacuate the silane from the desiccator and thus minimize the effectiveness of the treatment. STORAGE OF PROTEINS For long term storage (more than a few days), proteins are best kept at very low temperatures, either in a -80° C freezer, or under liquid nitrogen (-196° C). Because proteins themselves have some antifreeze and buffering capacities, they are best stored at high concentrations.

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