Pseudogenes: Functions and Protocols by Laura Poliseno

By Laura Poliseno

Providing a listing of equipment worthwhile either to people who desire to learn pseudogenes and to those that truly are looking to steer clear of their inadvertent detection, Pseudogenes: capabilities and Protocols explores concepts related to pseudogenic DNA, RNA, and peptides/proteins, as soon as believed to lack any performance, yet referred to now to be occupied with advanced regulatory circuits. After a couple of introductory chapters that review the features thus far attributed to pseudogenes, this thorough quantity delves into tools for pseudogene id, for the detection of pseudogene transcription and translation, and for the research of the services of pseudogenic RNA and proteins, in addition to the right way to keep away from pseudogene detection while the focal point of the learn is their hugely homologous parental opposite numbers. As a part of the hugely winning Methods in Molecular Biology sequence, chapters function the type of particular descriptions and implementation suggestion that guarantees profitable leads to the lab.

Authoritative and sensible, Pseudogenes: capabilities and Protocols will give a contribution to the excessive curiosity of the medical group towards pseudogenes, whereas stimulating the perception of pseudogene - based study tasks and delivering experimental protocols which can facilitate their execution.

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In the case of the eukaryotic organisms the number of functional genes is higher, up to 150 in Babesia bovis, which has 24 pseudogenes. The largest number of pseudogenes appears on T. brucei (up to 2,000) [29]. GC base pairs have been shown to be crucial for the gene-pseudogene recombination, by forming special 16 base pair guanine-rich fourstranded DNA structures. These structures, which have a special topology that is much wider than the normal B-DNA double helix, can act as recombination initiation sites [30].

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