Quantitative Applications of Mass Spectrometry by Pietro Traldi, Franco Magno, Irma Lavagnini, Roberta
By Pietro Traldi, Franco Magno, Irma Lavagnini, Roberta Seraglia
This article provides the data had to layout a profitable quantitative research utilizing mass spectrometric options presently on hand and extensively hired. it truly is dedicated to the researchers of other components, who use mass spectrometry as a detector compatible for the measurements in their curiosity. * an important publication for the training mass spectroscopist * a real 'how-to' textual content for the practitioner concentrating on quantification instead of instrumental layout and methods * up to date based textual content describing equipment, experimental technique, services and obstacles, with facts research and interpretation * Brings jointly fabric broadly dispersed within the pertinent literature into one exact resource * across the world well-known staff of authors
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Additional info for Quantitative Applications of Mass Spectrometry
In its ‘linear’ configuration it consists only of an ion source and a detector, between which a region under vacuum, without any field, is present. Ions with a m/z accelerated by the action of a field V acquire a speed v. 1). If, from this equation, we put in the v value: v ¼ ½ð2zV=mÞ1=2 ð1:12Þ it is easy to observe that ions of different m/z values exhibit different speeds, inversely proportional to the square root of their m/z values. e. t ¼ l/v, it follows that: t ¼ lðm=2zVÞ1=2 ð1:13Þ This equation shows that ions of different m/z value reach the detector at different times, proportional to the square root of their m/z value.
Dole, L. L. Mack, R. L. Hines, R. C. Mobley, L. D. Ferguson and M. B. Alice, J. Chem. , 49, 2240–2249 (1968). 13. J. V. Iribarne and B. A. Thomson, J. Chem. , 2287–2294 (1976). 14. P. Traldi and E. Marotta in Advances in Mass Spectrometry, Vol. 16, A. E. Ashcroft, G. Brenton and J. J. Monagan (Eds), Elsevier, Amsterdam (2004), pp. 275–293. 15. A. J. Dempster, J. Phys. , 11, 316–320 (1918). 16. F. W. Aston, Nature, 127, 813–820 (1931). 17. (a) J. H. Beynon in Mass Spectrometry and its Applications to Organic Chemistry, Elsevier, Amsterdam (1960), pp.
Of course the maximum value of resolution corresponds to very narrow slit width and consequently can be achieved in low sensitivity conditions. 3 Quadrupole Analysers Quadrupole mass filter20,21 and quadrupole ion trap22,23 are currently the mass analysers most widely employed. They were both developed by the Paul group (Nobel Prize for physics in 1989) at Bonn University. The theoretical data of the behaviour of an ion in a quadrupole electrical field is highly complex. Here only a picture of such behaviour is given in order to give the reader a view of what is happening inside these devices.