Quantitative chromatographic analysis by Thomas E Beesley; Benjamin Buglio; Raymond P W Scott

By Thomas E Beesley; Benjamin Buglio; Raymond P W Scott

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This sampling technique has been widely applied to many vapor-in-air problems, ranging from the analysis of the volatile components from blood and urine for diagnostic purposes to monitoring soil pollution, and combustion research. The technique is reliable, extremely sensitive, and can provide accurate proportional analysis. However, as the concentration of the vapor of each solute in the air will depend on its distribution coefficient between the sample matrix and air, the value for each will differ and thus absolute concentrations in the sample matrix can not be assayed.

The sample syringe is made to pierce the septum and then to dip into the sample solution. The appropriate amount is then withdrawn into the syringe. This procedure can be manual or, if the vial is situated in the carousel of an automatic sampler, can be actuated automatically. There is little to choose between the accuracies that can be obtained by the two methods, but temperature desorption of the sample is probably the simpler procedure. However, in using the temperature desorption technique, the thermal stability of the components of the mixture at the desorption temperature must always be considered.

Due to the relatively high pressures involved, and the relatively fragile nature of the membranes, they are usually required to be supported on an appropriate web structure to provide adequate mechanical strength. Both dialysis and ultra-filtration are relatively gentle processes and are useful for concentrating thermally labile materials that can not be heated. Of the two methods dialysis is more easily miniaturized and easier to automate and is probably in more common use than ultrafiltration.

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