Satellite DNA by Professor Dr. Thengiz Beridze (auth.)
By Professor Dr. Thengiz Beridze (auth.)
The association of genomes in greater organisms has been studied widely lately. With present achievements in gene engineering, it sort of feels really practical that they're going to be in particular transformed within the nearest destiny to provide new, economi cally useful types of animals and crops. The luck of those experiments will count enormously at the point of our wisdom of the structural gains of plant and animal DNAs. Comparative experiences of DNA from various organisms all started with discovery of its genetic value within the overdue 1950's. many years later it was once came across that nuclear DNA, the most garage of genetic information,~an encompass a number of fractions vary ing in a few actual and chemical homes. besides the "major" DNA, bearing the most load through the genotype functioning, the so-called satellite tv for pc DNAs have been stumbled on. T. G. Beridze, the writer of this publication, is without doubt one of the pioneers within the research of those remarkable DNAs. the result of his experiments with plant satellite tv for pc DNAs have primarily inspired the formation of our present rules on their constitution and prop erties.
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Extra info for Satellite DNA
0----, 560 . cifliilG C T TAT G T A C G GAT G C A AT A AT G U A11 G C T T A AT T C T T C T T G ~ ~ ~ ~ m m .!..! 8T T C TffiilG! AC G CA C C G TG TIAA G GICG TjA AA GILfG ~ m ~ T T C GIA A G GIG A GAG GA TIG G A AIC A GAT C C C G T A C G C A C G G GAG GAG C G C T C A C G Fig. 15. Nucleotide sequence of the pyrimidine-rich regions of Gecarcinus lateralis stDNA (LaMarca et al. 1981). The adjoining Ts or CTs are underlined. The sequences 5' ... CTTT d 5' ... CCTT d h" t b d GAAA ... 5' an GGAA ...
5' 5' ... AATATAT TTATATA ... 5' -60% -40%. The molecule consisting of pentamers is more homogeneous; the stDNA chain consisting of septamers is interspersed with GC pairs though with a lower frequency -less than one GC pair per 50 bp. 3-10· 10 6 daltons. The sequence digested by HaeIII is not included in the basic repeat structures of this satellite (Shen et al. 1976). 672, consists of two types of molecules with closely related repeats (Fry and Brutlag 1979). The molecule consisting oftandemly arranged pentamers comprises 85 % of all the DNA; the other molecule consists of septamer segments: 5' ...
Nucleotide sequence of the pyrimidine-rich regions of Gecarcinus lateralis stDNA (LaMarca et al. 1981). The adjoining Ts or CTs are underlined. The sequences 5' ... CTTT d 5' ... CCTT d h" t b d GAAA ... 5' an GGAA ... 2 Arthropods GACTCTGCCTCACACCGCCGACTGCTACGCAAGCCGCTATGCGGC ATGGCCTGTCGAAAAAACGAAAAC ---G G T-G-G-T-G ---G G T AT-G-G-T-G EXT : RU: TRU: EXT: RU: TRU: +++++++ +++++++ • . +++ 70 ATGCGTACGTTATTAGAATAACG TAAGAACAGG 70 C---I---G-ACAACAGCAAGAGCAGGAACAAGAAC 71 C G-ACAACAAGAAGAGCAGGAACAAGAAC + .