The Cartoon Guide to Genetics (Updated Edition) by Larry Gonick

By Larry Gonick

Have you ever requested yourself:

Are spliced genes kind of like mended Levis?

Watson and Crick? aren't they a workforce of British detectives?

Plant intercourse? Can they do that?

Is Genetic Mutation the identify of 1 of these heavy steel bands?

Asparagine? Which of the 4 meals teams is that in?

Then you wish The comic strip consultant to Genetics to give an explanation for the real recommendations of classical and smooth genetics—it's not just academic, it is humorous too!

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Natl. Acad. Sci. USA 81, 4275–4279. McGhee, J. D. and von Hippel, P. H. (1975) Formaldehyde as a probe of DNA structure. I. Reaction with exocyclic amino groups of DNA bases. Biochemistry 14, 1281–1296. McGhee, J. D. and von Hippel P. H. (1975) Formaldehyde as a probe of DNA structure. II. Reaction with endocyclic imino groups of DNA bases. Biochemistry 14, 1297–1303. Orlando, V. and Paro, R. (1993) Mapping Polycomb-repressed domains in the bithorax complex using in vivo formaldehyde cross-linked chromatin.

1. as follows: 1. Supplement the nuclear digest buffer with 250 mM sucrose. 2. Digest K562 nuclei at 5 mg/mL (DNA) using 300 U micrococcal nuclease (MNase)/mg DNA at 20°C for 2–5 min. The length of time and level of enzyme will vary considerably depending on cell type (see Note 1). 5 mM spermidine for magnesium chloride in preparation and digestion buffers to stabilize the nuclei (15) and minimize endonuclease and protease digestion. 3. Salt Fractionation Active sequences in a micrococcal nuclease-generated chromatin preparation can be enriched by salt precipitation of the H1/H5-containing chromatin (16,17).

1999). A developmental switch in H4 acetylation upstream of Xist plays a role in X chromosome inactivation. EMBO J. 18, 2897–2907. 13. , Cooke, N. , and Liebhaber, S. A. (2000). Targeted recruitment of histone acetyltransferase activity to a locus control region. J. Biol. Chem. 275, 13,827–13,834. 14. , Liebhaber, S. , and Cooke, N. E. (2000). Patterns of histone 14 acetylation suggest dual pathways for gene activation by a bifunctional locus control region. EMBO J. 19, 6814–6822. 15. Hewish, D.

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