The Chromatography of Steroids by I. E. Bush, R. K. Callow, P. N. Campbell and S. P. Datta

By I. E. Bush, R. K. Callow, P. N. Campbell and S. P. Datta (Auth.)

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Bate-Smith and Westall (1950) first emphasized the value of using RM units, and only the size of the molecules they studied places their work so late in the argument. They showed that a wide variety of natural polyphenolic compounds of the flavone type obeyed Martin's theory very closely indeed. For our purpose it is interesting to observe that these compounds were mainly C15 compounds with two fused rings and a third ring attached by a single bond permitting fairly free rotation (Fig. 7). OHN XH2 ' I II 0 FIG.

However, Barton, 1955). This is, of course, the ideal requirement for the application of the theoretical approach outlined in the previous chapter. 2. SPECIAL FEATURES OF THE CHROMATOGRAPHY OF STEROIDS The steroids are large, more or less slab-shaped, molecules with molecular weights ranging from about 270 to 1000. They are extraordinarily numerous and have a wide range of properties. They include for instance the extremely hydrophobic esters of the sterols, the androgens, the phenolic oestrogens, the adrenal cortical hormones and their metabolites, the sapogenins and cardiac aglycones, and also the water-soluble conjugated steroids such as the glycosides, bile salts, and glucuronosides (Fig.

However, Barton, 1955). This is, of course, the ideal requirement for the application of the theoretical approach outlined in the previous chapter. 2. SPECIAL FEATURES OF THE CHROMATOGRAPHY OF STEROIDS The steroids are large, more or less slab-shaped, molecules with molecular weights ranging from about 270 to 1000. They are extraordinarily numerous and have a wide range of properties. They include for instance the extremely hydrophobic esters of the sterols, the androgens, the phenolic oestrogens, the adrenal cortical hormones and their metabolites, the sapogenins and cardiac aglycones, and also the water-soluble conjugated steroids such as the glycosides, bile salts, and glucuronosides (Fig.

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